Interleukin-10-mediated inhibition of free radical generation in macrophages

Citation
S. Dokka et al., Interleukin-10-mediated inhibition of free radical generation in macrophages, AM J P-LUNG, 280(6), 2001, pp. L1196-L1202
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
ISSN journal
1040-0605 → ACNP
Volume
280
Issue
6
Year of publication
2001
Pages
L1196 - L1202
Database
ISI
SICI code
1040-0605(200106)280:6<L1196:IIOFRG>2.0.ZU;2-X
Abstract
Interleukin-10 (IL-10) is a pleiotropic cytokine that controls inflammatory processes by suppressing the production of proinflammatory cytokines that are known to be transcriptionally regulated by nuclear factor-kappaB (NF-ka ppaB). Although still controversial, IL-10 has been shown to inhibit NF-kap paB activation through a process that involves proteolytic degradation of i nhibitory subunit I kappaB-alpha. What is not known, however, is the mechan ism by which IL-10 exerts its effect on I kappaB-alpha degradation. The pre sent study investigates the possible role of reactive oxygen species (ROS) and their inhibition by IL-10 in NF-kappaB activation and I kappaB-alpha de gradation in macrophages. Treatment of the cells with lipopolysaccharide (L PS) caused activation of NF-kappaB and rapid proteolysis of I kappaB-alpha as determined by the electrophoretic mobility shift assay, gene transfectio n, and Western blot. IL-10 pretreatment inhibited both NF-kappaB activation and I kappaB-alpha degradation. Both of these processes were also inhibite d by ROS scavengers, catalase (H2O2 scavenger), and sodium formate (. OH sc avenger) but were minimally affected by superoxide dismutase (O-2(-) scaven ger). These results suggests that . OH radicals, formed by an H2O2-dependen t, metal-catalyzed Fenton reaction, play a major role in this process. Elec tron spin resonance studies confirmed the formation of . OH radicals in LPS -treated cells. Addition of IL-10 inhibited both I kappaB-alpha degradation and generation of . OH radicals in response to LPS stimulation. These resu lts demonstrate, for the first time, direct evidence for the role of IL-10 in ROS-dependent NF-kappaB activation.