Bafilomycin A(1) inhibits rhinovirus infection in human airway epithelium:effects on endosome and ICAM-1

Citation
T. Suzuki et al., Bafilomycin A(1) inhibits rhinovirus infection in human airway epithelium:effects on endosome and ICAM-1, AM J P-LUNG, 280(6), 2001, pp. L1115-L1127
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
ISSN journal
1040-0605 → ACNP
Volume
280
Issue
6
Year of publication
2001
Pages
L1115 - L1127
Database
ISI
SICI code
1040-0605(200106)280:6<L1115:BAIRII>2.0.ZU;2-P
Abstract
To examine the effects of bafilomycin A(1), a blocker of vacuolar H+-ATPase , on rhinovirus (RV) infection in the airway epithelium, primary cultures o f human tracheal epithelial cells were infected with RV14. Viral infection was confirmed by showing that viral RNA in the infected cells and the viral titers in the supernatants of infected cells increased with time. RV14 inf ection upregulated the production of cytokines and mRNA of intercellular ad hesion molecule (ICAM)-1 in epithelial cells. Bafilomycin A(1) reduced the viral titers of RV14 and inhibited the production of cytokines and ICAM-1 b efore and after RV14 infection. Bafilomycin A(1) reduced susceptibility of epithelial cells to RV14 infection. RV14 increased activated nuclear factor -kappaB in the cells, and bafilomycin A(1) reduced the activated nuclear fa ctor-kappaB. Bafilomycin A(1) decreased the number of acidic endosomes in t he epithelial cells. These results suggest that bafilomycin A(1) may inhibi t infection by RV14 by not only blocking RV RNA entry into the endosomes bu t also reducing ICAM-1 expression in the epithelial cells. Bafilomycin A(1) may therefore modulate airway inflammation after RV infection.