The lacZ gene under the control of the 7 kb of human dystrophin muscle specific promoter is expressed in cardiac muscle but not in adult skeletal muscle in transgenic mice

Citation
M. Ogawa et al., The lacZ gene under the control of the 7 kb of human dystrophin muscle specific promoter is expressed in cardiac muscle but not in adult skeletal muscle in transgenic mice, NEUROMUSC D, 11(3), 2001, pp. 244-250
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROMUSCULAR DISORDERS
ISSN journal
0960-8966 → ACNP
Volume
11
Issue
3
Year of publication
2001
Pages
244 - 250
Database
ISI
SICI code
0960-8966(200104)11:3<244:TLGUTC>2.0.ZU;2-3
Abstract
In previous transgenic studies, we reported a 0.9 kb fragment from a mouse dystrophin muscle promoter that contains the regulatory elements required f or expression of dystrophin only in the right heart. In this study, to furt her characterize the regulation of muscle type of promoter. we analyzed pro moter activity and tissue specificity using a total 14 kb fragment around t he human dystrophin muscular-specific exon I in vitro, and in vivo. In vitr o analysis showed that the lacZ construct of the 7 kb promoter and 7 kb int ron 1 was expressed 2.5 times as strong as the lacZ construct of only the 7 kb promoter in C2/4 myotubes. In vivo analysis revealed expression of both constructs in the whole heart. skeletal muscle and vascular smooth muscle in embryos. However. in adults, the expression in skeletal muscle disappear ed. We conclude that the 7 kb upstream region and the 7 kb intronic region included responsible elements for the expression in the heart, but not in s keletal muscle in vivo. It is possible that a strong enhancer element for s keletal muscle exists in some other region, (C) 2001 Elsevier Science B.V. All rights reserved.