Vascular differences detected by MRI for metastatic versus nonmetastatic breast and prostate cancer xenografts

Citation
Zm. Bhujwalla et al., Vascular differences detected by MRI for metastatic versus nonmetastatic breast and prostate cancer xenografts, NEOPLASIA, 3(2), 2001, pp. 143-153
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
NEOPLASIA
ISSN journal
1522-8002 → ACNP
Volume
3
Issue
2
Year of publication
2001
Pages
143 - 153
Database
ISI
SICI code
1522-8002(200103/04)3:2<143:VDDBMF>2.0.ZU;2-I
Abstract
Several studies have linked vascular density, identified in histologic sect ions, to "metastatic risk." Functional information of the vasculature, not readily available from histologic sections, can be obtained with contrast-e nhanced MRI to exploit for therapy or metastasis prevention. Our aims were to determine if human breast and prostate cancer xenografts preselected for differences in invasive and metastatic characteristics established corresp ondingly different Vascular Volume and permeability, quantified here with n oninvasive MRI of the intravascular contrast agent albumin-GdDTPA. Tumor va scular volume and permeability of human breast and prostate cancer xenograf ts were characterized using MRI, Parallel studies confirmed the invasive be havior of these cell lines. Vascular endothelial growth factor (VEGF) expre ssion in the cell lines was measured using ELISA and Western blots. Metasta sis to the lungs was evaluated with spontaneous as well as experimental ass ay. Metastatic tumors formed vasculature with significantly higher permeabi lity or vascular volume (P<.05, two-sided unpaired t test). The permeabilit y profile matched VEGF expression. Within tumors, regions of high vascular Volume usually exhibited low permeability whereas regions of low vascular v olume exhibited high permeability. We observed that although invasion was n ecessary, without adequate vascularization it was not sufficient for metast asis to occur.