Detection of MYCN gene amplification in neuroblastoma by fluorescence in situ hybridization: A pediatric oncology group study

Citation
P. Mathew et al., Detection of MYCN gene amplification in neuroblastoma by fluorescence in situ hybridization: A pediatric oncology group study, NEOPLASIA, 3(2), 2001, pp. 105-109
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
NEOPLASIA
ISSN journal
1522-8002 → ACNP
Volume
3
Issue
2
Year of publication
2001
Pages
105 - 109
Database
ISI
SICI code
1522-8002(200103/04)3:2<105:DOMGAI>2.0.ZU;2-T
Abstract
To assess the utility of fluorescence in situ hybridization (FISH) for anal ysis of MYCN gene amplification in neuroblastoma, we compared this assay wi th Southern blot analysis using tumor specimens collected from 232 patients with presenting characteristics typical of this disease. The FISH techniqu e identified MYCN amplification in 47 cases, compared with 39 by Southern b lotting, thus increasing the total number of positive cases by 21%. The maj or cause of discordancy was a low fraction of tumor cells (less than or equ al to 30% replacement) in clinical specimens, which prevented an accurate e stimate of MYCN copy number by Southern blotting. With FISH, by contrast, i t was possible to analyze multiple interphase nuclei of tumor cells, regard less of the proportion of normal peripheral blood, bone marrow, or stromal cells in clinical samples. Thus, FISH could be performed accurately with ve ry small numbers of tumor cells from touch preparations of needle biopsies, Moreover, this procedure allowed us to discern the heterogeneous pattern o f MYCN amplification that is characteristic of neuroblastoma. We conclude t hat FISH improves the detection of MYCN gene amplification in childhood neu roblastomas in a clinical setting, thus facilitating therapeutic decisions based on the presence or absence of this prognostically important biologic marker.