Rod and cone degeneration in the rd mouse is p53 independent

Citation
J. Wu et al., Rod and cone degeneration in the rd mouse is p53 independent, MOL VIS, 7(15), 2001, pp. 101-106
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
MOLECULAR VISION
ISSN journal
1090-0535 → ACNP
Volume
7
Issue
15
Year of publication
2001
Pages
101 - 106
Database
ISI
SICI code
1090-0535(20010426)7:15<101:RACDIT>2.0.ZU;2-C
Abstract
PURPOSE: To determine whether p53 is required for the death of rod and cone photoreceptors in rd mice, a model of human retinitis pigmentosa, and/or f or the natural degeneration of inner nuclear layer (INL) cells in the devel oping retina. METHODS: Rod photoreceptor and INL apoptosis was assessed by TUNEL staining of mouse sagittal sections from post natal day (P) 10, 13, 15, 17, and 20 day p53+/+ and p53-/- rd retinas. Cone photoreceptor survival was measured by counting the total number of peanut agglutinin (PNA) positive cells in e ighty four 0.25 mm x 0.25 mm bins in each eye, distributed equally across t he four quadrants of whole mount retinas from 3 month old p53+/+ and p53-/- rd retinas. RESULTS: Both the kinetics of rod and INL cell death as well as the surviva l of cones were essentially unaffected by the absence of p53. CONCLUSIONS: Despite established links with retinal apoptosis, p53 is not e ssential for rod or cone cell degeneration in the rd mouse or for the elimi nation of bipolar and Muller cells during late retinal development.