Rapid induction of mRNAs for PC3 by partial hepatectomy and epidermal growth factor

Citation
G. Wang et al., Rapid induction of mRNAs for PC3 by partial hepatectomy and epidermal growth factor, CHIN SCI B, 46(7), 2001, pp. 571-574
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
1001-6538 → ACNP
Volume
46
Issue
7
Year of publication
2001
Pages
571 - 574
Database
ISI
SICI code
1001-6538(200104)46:7<571:RIOMFP>2.0.ZU;2-O
Abstract
The expression of immediate early gene plays a pivotal role in rat hepatocy te proliferation from G(0) to G(1) phases and the progression through G(1) phase of the cell cycle within several hours after 2/3 hepatectomy, We inve stigated the different gene expressions within 1 h after 2/3 hepatectomy by representational difference analysis. Sequence analysis indicated that PC3 induced by NGF was a kind of immediate early gene and might be correlated with liver regeneration. Moreover, we Pound that 2/3 hepatectomy could indu ce the expressing of PC3 mRNA by Northern blot with a peak 1-2 h after surg ery. In primary cultures of rat hepatocytes, addition of EGF resulted in ra pid and transient Induction of PC3 mRNA, It was first reported that PC3 gen e belongs to immediate early gene associated with liver regeneration.