Isolation and characterization of the third gene encoding a 16.9 kDa classI low-molecular-mass heat shock protein, Oshsp 16.9C, in rice

Citation
Pfl. Chang et al., Isolation and characterization of the third gene encoding a 16.9 kDa classI low-molecular-mass heat shock protein, Oshsp 16.9C, in rice, BOTAN B A S, 42(2), 2001, pp. 85-92
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
BOTANICAL BULLETIN OF ACADEMIA SINICA
ISSN journal
0006-8063 → ACNP
Volume
42
Issue
2
Year of publication
2001
Pages
85 - 92
Database
ISI
SICI code
0006-8063(200104)42:2<85:IACOTT>2.0.ZU;2-Q
Abstract
Using rice cDNA pTS1 encoding a 16.9 kDa heat shock protein (HSP) as a prob e for Southern hybridization analysis, we observed five prominent bands of 9.5, 5.9, 3.4, 2.5, and 1.7 kb in the Eco RI digests of rice genomic DNA an d found them to contain six individual genes. The 5.9 kb DNA fragment was f urther digested with Hind m to generate three fragments of 3.5, 1.7 and 0.7 kb, and we found, using pTS1 cDNA as a probe, the 3.5 kb fragment containe d a putative low-molecular-mass (LMM) HSP gene. The DNA sequencing of 3.5 k b fragment revealed encoding of a presumptive 16.9 kDa HSP (149 amino acid residues) with predicted pi value of 6.42. The nucleotide sequence of this gene was highly homologous to the coding regions of two rice class I LMM HS P genes, Oshsp, 16.9A and Oshsp16.9B, sharing 93.1% and 94.3% sequence iden tity, respectively, as published previously in our laboratory. The deduced amino acid sequence of this gene is similar to those of the Oshsp16.9A and Oshsp16.9B genes with a difference of only ii and 10 amino acids, respectiv ely. It was hence designated as Oshsp16.9C (accession no. U81385). We used 3' UTRs (untranslated regions) for analysis of Oshsp16.9C gene expression s ince the 3' UTRs of these three genes showed Very low sequence homology.