Replication of Chilo iridescent virus in the cotton boll weevil, Anthonomus grandis, and development of an infectivity assay

Citation
Cw. Henderson et al., Replication of Chilo iridescent virus in the cotton boll weevil, Anthonomus grandis, and development of an infectivity assay, ARCH VIROL, 146(4), 2001, pp. 767-775
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
0304-8608 → ACNP
Volume
146
Issue
4
Year of publication
2001
Pages
767 - 775
Database
ISI
SICI code
0304-8608(2001)146:4<767:ROCIVI>2.0.ZU;2-V
Abstract
The boll weevil, Anthonomus grandis Boheman, is a devastating pest of cotto n. Chemical pesticides are problematic due to relative lack of target speci ficity and resistance. Microbial pesticides may provide viable alternatives because of their narrow host range. Chilo iridescent virus (CIV) is the ty pe species for genus Iridovirus, family Iridoviridae: large, icosahedral cy toplasmic viruses containing a double-stranded DNA genome. Earlier work sug gested that CIV replicated in the bell weevil; however, efficiency or produ ction of infectious virus was not established. We showed that CIV undergoes a productive cycle in A. grandis. CIV DNA levels in bell weevil pupae incr eased significantly from 0 to 3 days post infection. Moreover, virogenic st romata and complete virus particles were observed in the cytoplasm by 7 day s. An endpoint dilution assay using viral DNA replication as indicator sugg ested a 10(5)-fold increase in infectious virus titer over 7 days. This is the first such demonstration in larval infections with genus Iridovirus. Ou r study establishes that CIV undergoes a productive cycle in the bell weevi l and provides an important and useful model system for replication at the or,organismal level. These results have important implications for the pote ntial of CIV and its components in bell weevil control.