Mechanisms underlying the acquisition of resistance to octanoic-acid-induced-death following exposure of Saccharomyces cerevisiae to mild stress imposed by octanoic acid or ethanol

Citation
Mg. Cabral et al., Mechanisms underlying the acquisition of resistance to octanoic-acid-induced-death following exposure of Saccharomyces cerevisiae to mild stress imposed by octanoic acid or ethanol, ARCH MICROB, 175(4), 2001, pp. 301-307
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
175
Issue
4
Year of publication
2001
Pages
301 - 307
Database
ISI
SICI code
0302-8933(200104)175:4<301:MUTAOR>2.0.ZU;2-Q
Abstract
Acquisition of resistance to lethal concentrations of octanoic acid was ind uced in cells of Saccharomyces cerevisiae grown in the presence of subletha l concentrations of this lipophilic acid or following rapid exposure (1 h) of unadapted yeast cells to mild stress imposed by the same acid. Experimen tal evidence indicated that the referred adaptation involved de novo protei n synthesis, presumably due to the rapid induction of a plasma membrane tra nsporter which mediates the active efflux of octanoate out of the cell. Rap id exposure of cells to mild ethanol stress also led to increased resistanc e to lethal concentrations of octanoic acid. This cross-resistance to octan oic-acid-induced death was below the level of resistance induced by mild oc tanoic acid stress and did not involve induction of the active expulsion of octanoate out of the cell. However, the rapid exposure of yeast cells to o ctanoic acid or ethanol led to the activation of plasma membrane H+-ATPase. The physiological role of the two stress responses examined during the pre sent study, namely, the active efflux of octanoate specifically induced by octanoic acid and the stimulation of plasma membrane H+-ATPase activity, is discussed.