Transformation of azuki bean by Agrobacterium tumefaciens

Citation
T. Yamada et al., Transformation of azuki bean by Agrobacterium tumefaciens, PL CELL TIS, 64(1), 2001, pp. 47-54
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
PLANT CELL TISSUE AND ORGAN CULTURE
ISSN journal
0167-6857 → ACNP
Volume
64
Issue
1
Year of publication
2001
Pages
47 - 54
Database
ISI
SICI code
0167-6857(2001)64:1<47:TOABBA>2.0.ZU;2-8
Abstract
Stable transformation and regeneration was developed for a grain legume, az uki bean (Vigna angularis Willd. Ohwi & Ohashi). Two constructs containing the neomycin phosphotransferase II gene (nptII) and either the beta -glucur onidase (GUS) gene or the modified green fluorescent protein [sGFP(S65T)] g ene were introduced independently via Agrobacterium tumefaciens-mediated tr ansformation. After 2 days of co-cultivation on MS medium supplemented with 100 muM acetosyringone and 10 mg 1(-1) 6-benzyladenine, seedling epicotyl explants were placed on regeneration medium containing 100 mg 1(-1) kanamyc in. Adventitious shoots developing from explant calli were excised onto roo ting medium containing 100 mg 1(-1) kanamycin. Rooted shoots were excised a nd repeatedly selected on the same medium containing kanamycin. Surviving p lants were transferred to soil and grown in a green house to produce viable seeds. This process took 5 to 7 months after co-cultivation. Molecular ana lysis confirmed the stable integration and expression of foreign genes.