Interleukin-1 beta induces the expression of lipocortin 1 mRNA in culturedrat cortical astrocytes

Citation
T. Miyachi et al., Interleukin-1 beta induces the expression of lipocortin 1 mRNA in culturedrat cortical astrocytes, NEUROSCI RE, 40(1), 2001, pp. 53-60
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE RESEARCH
ISSN journal
0168-0102 → ACNP
Volume
40
Issue
1
Year of publication
2001
Pages
53 - 60
Database
ISI
SICI code
0168-0102(200105)40:1<53:IBITEO>2.0.ZU;2-7
Abstract
Lipocortin 1 (LC1) has been shown to increase in neuronal damage and act as a neuroprotectant and a neurotrophic factor. IL-I beta acts as a mediator of inflammation and has been reported as ii potent inducer of various neuro trophic factors including nerve growth factor and fibroblast growth factor. In this study. we investigated the relationship between LC1 and IL-1 beta in cultured rat astrocytes. Time- and dose-dependent experiments of IL-IP o n rat cortical astrocytes in culture revealed that the expression of LC1 mR NA was significantly augmented by IL-1 beta at 8 h, 10 ng/ml. In addition, IL-1 beta evoked an extracellular secretion of LC1 without its cytotoxic ef fects. The effect of IL-1 beta was completely abolished when we treated cel ls with inhibitor of mitogen-activated protein kinases (MAPKs) (PD98059) (2 5 muM), phospholipase A(2) inhibitor mepacrine (30 muM) and protein synthes is inhibitor cycloheximide (CHX) (10 mug/ml). This suggests that induction of LC1 by IL-1 beta is through a MAPKs and phospholipaseA(2) pathway and re quires protein synthesis. These results indicate that IL-1 beta released in the central nervous system (CNS) injury can stimulate the transcription of the LC1 gene. Subsequent synthesis and release of LC1 may provide trophic support to neurons and modulate the action of IL-1 beta in brain damage. (C ) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.