Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida

Citation
L. Fioretti et al., Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida, NEMATOLOGY, 3, 2001, pp. 45-54
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences
Journal title
NEMATOLOGY
ISSN journal
1388-5545 → ACNP
Volume
3
Year of publication
2001
Part
1
Pages
45 - 54
Database
ISI
SICI code
1388-5545(2001)3:<45:IALOAA>2.0.ZU;2-2
Abstract
A full length cDNA clone was isolated by screening a mixed stage Globodera pallida expression library using the monospecific polyclonal antibody IACR- PC320. Sequence comparison indicated that the predicted 332 amino acid prot ein encoded by the cDNA was a member of the annexin gene family named gp-ne x and very similar to annexins found in other nematode species. Annexins ar e calcium-dependent phospholipid binding proteins characterised by four rep eated domains approximately 70 amino acids in length. The predicted amino a cid sequence of the protein did not contain an N-terminal secretion signal peptide; however, the protein was shown to be present in excretory/secretor y products from G. pallida second stage juveniles treated with the neurotra nsmitter 5 methoxy-N, N dimethyl tryptamine. Gp-nex encodes a protein of 35 kDa and was immunolocalised in the amphids, genital primordium and in the constraining muscles above and below the metacorpus pump chamber of G. pall ida second stage juveniles. It is also present in eggs and adult females of the two species of the potato cyst nematodes.