Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat

Citation
L. Edvinsson et al., Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat, MICROSC RES, 53(3), 2001, pp. 221-228
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
MICROSCOPY RESEARCH AND TECHNIQUE
ISSN journal
1059-910X → ACNP
Volume
53
Issue
3
Year of publication
2001
Pages
221 - 228
Database
ISI
SICI code
1059-910X(20010501)53:3<221:OACONO>2.0.ZU;2-I
Abstract
The origin of perivascular nerve fibres storing nitric oxide synthase (NOS) and co-localisation with perivascular neuropeptides were examined in the r at middle cerebral artery (MCA) by retrograde tracing with True Blue (TB) i n combination with immunocytochemistry. Application of TB to the proximal p art of the middle cerebral artery labelled nerve cell bodies ipsilaterally in the trigeminal, sphenopalatine, otic, and superior cervical ganglia. A f ew labelled cell bodies were seen contralaterally, suggesting bilateral inn ervation. In the parasympathetic sphenopalatine and otic ganglia, numerous TB-labelled cell bodies contained neuronal NOS (C- and N-terminal), vasoact ive intestinal peptide (VIP), and pituitary adenylate cyclase activating pe ptide (PACAP). In the trigeminal ganglion, almost all TB-labelled cell bodi es contained calcitonin generelated peptide (CGRP) but only a few cells con tained NOS. In the superior cervical ganglion, the majority of the TB-label led nerve cells contained neuropeptide Y (NPY) but none of them contained N OS. Removal of the ipsilateral sphenopalatine ganglion caused a slight redu ction in the number of perivascular VIP-, PACAP-, and NOS-containing fibres after 3 days in the MCA while there was no difference at 2 and 4 weeks aft er the denervation as compared to control. This indicates that the parasymp athetic VIP-, PACAP-, and NOS-immunoreactive nerve fibres in the rat MCA or iginate from several sources. (C) 2001 Wiley-Liss, Inc.