Real-time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy

Citation
U. Pilatus et al., Real-time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy, MAGN RES M, 45(5), 2001, pp. 749-755
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Radiology ,Nuclear Medicine & Imaging","Medical Research Diagnosis & Treatment
Journal title
MAGNETIC RESONANCE IN MEDICINE
ISSN journal
0740-3194 → ACNP
Volume
45
Issue
5
Year of publication
2001
Pages
749 - 755
Database
ISI
SICI code
0740-3194(200105)45:5<749:RMOCOC>2.0.ZU;2-C
Abstract
Changes in molecular expression or apoptotic behavior, induced by malignant transformation or anticancer treatment, are frequently reflected in cellul ar metabolism and oxygen consumption. A technique to monitor oxygen consump tion, cell physiology, and metabolism noninvasively would provide a better understanding of interactions between molecular changes and metabolism in m alignant transformation and following cancer treatment. Such a system was d eveloped in this study by adapting multinuclear MRI and spectroscopic techn iques to an isolated cell perfusion system. The system was evaluated by stu dying the effects of two agents, carbonyl cyanide m-chlorophenylhydrazone ( CCCP) which is an uncoupler of oxidative phosphorylation, and antimycin, an inhibitor of oxidative phosphorylation, on the oxygen consumption and meta bolism of MCF-7 and MatLyLu cancer cell lines. (C) 2001 Wiley-Liss, Inc.