M. Zenmyo et al., Transcriptional activation of p21 by vitamin D-3 or vitamin K-2 leads to differentiation of p53-deficient MG-63 osteosarcoma cells, HUMAN PATH, 32(4), 2001, pp. 410-416
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
p21 (WAF1/CIP1) is a downstream effector of p53 and mediates growth arrest
by inhibiting the action of G(1) cyclin-dependent kinases. However, it has
been reported that the p21 expression was triggered by multiple differentia
tion-inducing agents by a p53-independent pathway. These agents induced exp
ression of p21 by binding to specific DNA elements and modulating transcrip
tional initiation. We demonstrated that the gene encoding p21 was not only
a vitamin D-3 target gene but also vitamin K-2 target gene in the cells and
that their differentiation was well related to the transcriptional activat
ion of the p21 gene. Transient overexpression of p21, using adenovirus-driv
en p21 expression plasmid, in MG-63 cells in the absence of vitamins D-3 an
d K-2 resulted in their differentiation. The transcriptional activation of
p21 by vitamin D-3 or vitamin K-2 in p55-deficient osteosarcoma cells demon
strated the p53-independent role of p21 in human osseous differentiation.