Regulation of tubular cell MCP-1 production by intracellular ions: A role for sodium & calcium

Citation
Dch. Harris et al., Regulation of tubular cell MCP-1 production by intracellular ions: A role for sodium & calcium, EXP NEPHROL, 9(3), 2001, pp. 205-213
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
EXPERIMENTAL NEPHROLOGY
ISSN journal
1018-7782 → ACNP
Volume
9
Issue
3
Year of publication
2001
Pages
205 - 213
Database
ISI
SICI code
1018-7782(2001)9:3<205:ROTCMP>2.0.ZU;2-K
Abstract
Proximal tubule cells (PTC) in chronic renal disease produce chemokines whi ch cause renal interstitial inflammation, and also transport more Na+ than normal. To investigate whether these two events might be related, monocyte chemoattractant protein-1 (MCP-1) production was examined in rat PTC in pri mary culture. Amiloride reduced, while ouabain increased levels of MCP-1 mR NA and protein. Amiloride reduced MCP-1 in cells stimulated with ouabain, l ipopolysaccharide (LPS) or albumin. Intracellular Na+ rose with ouabain, bu t not LPS or albumin. Effects of amiloride, ouabain, LPS and albumin were a brogated by sodium-free but not chloride-free culture medium, and were not explained by changes in intracellular pH. Intracellular Ca2+ and MCP-1 mRNA levels in unstimulated cells, and cells stimulated with ouabain, LPS or al bumin. Thus, amiloride and ouabain may alter tubular cell MCP-1 by changing intracellular Na+, with secondary changes in intracellular Ca2+, whereas s timulation by LPS and albumin may involve Ca2+ directly. Copyright (C) 2001 S. Karger AG, Basel.