Active Ras induces heterodimerization of cRaf and BRaf

Citation
Ck. Weber et al., Active Ras induces heterodimerization of cRaf and BRaf, CANCER RES, 61(9), 2001, pp. 3595-3598
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER RESEARCH
ISSN journal
0008-5472 → ACNP
Volume
61
Issue
9
Year of publication
2001
Pages
3595 - 3598
Database
ISI
SICI code
0008-5472(20010501)61:9<3595:ARIHOC>2.0.ZU;2-6
Abstract
Growth factor-induced signalling leads to activation of members of the Ras family and subsequent stimulation of different Raf isoforms, Within the mec hanism of Raf activation, two isoforms of Raf, cRaf and BRaf, may cooperate . We investigated the relationship between cRaf and BRaf and found that act ive Ras induced heterodimerization of cRaf and BRaf, an effect that was dep endent on the serine residue at position 621 of cRaf. Moreover, we also fou nd that cRaf COOH-terminus constitutively associated with BRaf, whereas the NH2 terminus did not, even in the presence of active Ras, These data sugge st that Ras induces the cRaF-BRaf complex formation through the exposure of 14-3-3 binding sites in the COOH-terminus of cRaf, Thus, Ras-induced cRaf- Braf heterodimerization may explain the observed cooperativity of cRaf and BRaf in cells responding to growth factor signals.