A genetically tractable model of human glioma formation

Citation
Jn. Rich et al., A genetically tractable model of human glioma formation, CANCER RES, 61(9), 2001, pp. 3556-3560
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER RESEARCH
ISSN journal
0008-5472 → ACNP
Volume
61
Issue
9
Year of publication
2001
Pages
3556 - 3560
Database
ISI
SICI code
0008-5472(20010501)61:9<3556:AGTMOH>2.0.ZU;2-9
Abstract
Gliomas remain one of the deadliest forms of cancer. Improved therapeutics will require a better understanding of the molecular nature of these tumors . We, therefore, mimicked the most common genetic changes found in grade II I-IV gliomas, disruption of the p53 and RE pathways and activation of telom ere maintenance and independence from growth factors, through the ectopic e xpression of the SV40 T/t-Ag oncogene, an oncogenic form of H-ras (H-ras(V1 2G)), and the human telomerase catalytic subunit hTERT in normal human astr ocytes. The resulting cells displayed many of the hallmarks of grade III-IV gliomas, including greatly expanded life span and growth in soft agar and, most importantly, were tumorigenic with pathology consistent with grade II I-IV neuroectodermal tumors in mice. This model system will, for the first time, allow the biological significance of selected genetic alterations to be studied in human gliomas.