Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser-capture microdissection of tumor tissues andnormal epithelia

Citation
O. Kitahara et al., Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser-capture microdissection of tumor tissues andnormal epithelia, CANCER RES, 61(9), 2001, pp. 3544-3549
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER RESEARCH
ISSN journal
0008-5472 → ACNP
Volume
61
Issue
9
Year of publication
2001
Pages
3544 - 3549
Database
ISI
SICI code
0008-5472(20010501)61:9<3544:AOGEDC>2.0.ZU;2-G
Abstract
To identify a set of genes involved in the development of colorectal carcin ogenesis, we compared expression profiles of colorectal cancer cells from e ight tumors with corresponding noncancerous colonic epithelia using a DNA m icroarray consisting of 9216 human genes. These cell populations had been r endered homogeneous by laser-capture microdissection. Expression change in more than half of the tumors was observed for 235 genes, i.e., 44 up-regula ted and 191 down-regulated genes. The differentially expressed genes includ e those associated with signal transduction, metabolizing enzymes, producti on of reactive oxygen species, cell cycle, transcription, mitosis, and apop tosis, Subsequent examination of 10 genes (five up-regulated and five down- regulated) by semiquantitative reverse transcription-PCR using the eight tu mors together with an additional 12 samples substantiated the reliability o f our analysis. The extensive list of genes identified in these experiments provides a large body of potentially valuable information of colorectal ca rcinogenesis and represents a source of novel targets for cancer therapy.