Detection of truncated midkine in Wilms' tumor by a monoclonal antibody against human recombinant truncated midkine

Citation
S. Paul et al., Detection of truncated midkine in Wilms' tumor by a monoclonal antibody against human recombinant truncated midkine, CANCER LETT, 163(2), 2001, pp. 245-251
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER LETTERS
ISSN journal
0304-3835 → ACNP
Volume
163
Issue
2
Year of publication
2001
Pages
245 - 251
Database
ISI
SICI code
0304-3835(20010226)163:2<245:DOTMIW>2.0.ZU;2-R
Abstract
Although the expression of a truncated midkine (tMK) mRNA has been detected in many cancer cells, the tMK protein itself has not yet been identified. The expression, purification and characterization of human recombinant tMK were described in the former report [1]. A mouse hybridoma cell line produc ing an IgG(2b) monoclonal antibody (mab) against purified recombinant tMK w as established. This anti-tMK mab did not cross react with synthetic full l ength (or c-half) human midkine. A putative native tMK was identified in G4 01 cells using this mab, and showed the same apparent Mw as the recombinant tMK in SDS-PAGE. This mab was also used in an immunohistochemical study to evaluate the expression of tMK in Wilms' tumor cell line, G401 cells, as w ell as in Wilms' tumor patient specimens. G401 cells and all Wilms' tumor p atient specimens immunoreacted with this anti-tMK mab. We conclude that Wil ms' tumor cells express tMK and that this mab is useful for the detection o f tMK in the Wilms' tumor. (C) 2001 Elsevier Science Ireland Ltd. All right s reserved.