Frequent c-Ha-ras gene mutations in rat mammary carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine

Citation
N. Hokaiwado et al., Frequent c-Ha-ras gene mutations in rat mammary carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, CANCER LETT, 163(2), 2001, pp. 187-190
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER LETTERS
ISSN journal
0304-3835 → ACNP
Volume
163
Issue
2
Year of publication
2001
Pages
187 - 190
Database
ISI
SICI code
0304-3835(20010226)163:2<187:FCGMIR>2.0.ZU;2-2
Abstract
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), the most abundant h eterocyclic amine in cooked meat and fish, is carcinogenic to the mammary g lands of rats. Mutations in the H-ras gene were here examined in PhIP-induc ed mammary tumors of female F344 rats by the polymerase chain reaction foll owed by single strand conformation polymorphism analysis (PCR-SSCP) and res triction enzyme length polymorphism analysis (RFLP). Mutations in codon 12 of the H-ras gene were detected in four out of 13 tumors by PCR-SSCP. Three of them were GGA to GAA, and one was GGA to GTA. However, by RFLP analysis , four additional mutations in codon 13 were also detected in the same samp les. Two had a GGC to CGC mutation, and the other shifts were GGC to GAC an d GGC to GTC. Therefore, overall eight out of 13 cases had H-ras gene mutat ions. These results indicate that changes in H-ras function may play import ant roles in PhIP-induced mammary carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.