Muc1 and glycan expression in the oviduct and endometrium of a new world monkey, Cebus apella

Citation
Cjp. Jones et al., Muc1 and glycan expression in the oviduct and endometrium of a new world monkey, Cebus apella, BIOL REPROD, 64(5), 2001, pp. 1535-1544
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
BIOLOGY OF REPRODUCTION
ISSN journal
0006-3363 → ACNP
Volume
64
Issue
5
Year of publication
2001
Pages
1535 - 1544
Database
ISI
SICI code
0006-3363(200105)64:5<1535:MAGEIT>2.0.ZU;2-7
Abstract
Cebus apella is a New World monkey that has a menstrual cycle of 18-23 days with implantation at approximately luteal Day 5, The aim of this study was to characterize by lectin- and antibody-labeling the distribution of Mud a nd associated glycans on the endometrial and oviductal epithelium during th e luteal phase of the cycle, Endometrial histology showed a thin endometriu m, with glands extending deeply into the myometrium, No obvious evidence of secretory differentiation in cells of either the superficial or the basal segments of glands could be obtained using a panel of antibodies and lectin s that marked epithelial glycoprotein, and glycosylation changes observed i n some other primate endometrial cycles were not observed in this study, An tibodies to human MUC1 were shown to cross-react with C, apella, and Mud wa s localized to the apical epithelial surfaces of both the endometrial and t he tubal epithelium, with stronger expression in the latter. Again, no cycl ic changes were noted, Antibodies specific to the isoform Muc1/Sec showed s trong staining at the apical tubal epithelium, but no reactivity was detect able in the luminal epithelium of the uterus. This observation suggests dif ferences between the two glycocalyces and could help to explain why C, apel la embryos do not implant in this location.