Effect of age and breeding season on the developmental capacity of oocytesfrom unstimulated and follicle-stimulating hormone-stimulated rhesus monkeys

Citation
P. Zheng et al., Effect of age and breeding season on the developmental capacity of oocytesfrom unstimulated and follicle-stimulating hormone-stimulated rhesus monkeys, BIOL REPROD, 64(5), 2001, pp. 1417-1421
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
BIOLOGY OF REPRODUCTION
ISSN journal
0006-3363 → ACNP
Volume
64
Issue
5
Year of publication
2001
Pages
1417 - 1421
Database
ISI
SICI code
0006-3363(200105)64:5<1417:EOAABS>2.0.ZU;2-6
Abstract
Effects of age and season on the developmental capacity of oocytes from uns timulated and FSH-stimulated rhesus monkeys were examined, Immature cumulus -oocyte complexes were matured in vitro in modified CMRL-1066 medium contai ning 20% bovine calf serum and subjected to in vitro fertilization followed by embryo culture. After fertilization, ova from unstimulated prepubertal monkeys displayed lower development to morula (4%) than those from unstimul ated adult females (18% in breeding season and 22% in nonbreeding season). No developmental difference was found between ova from unstimulated adult m onkeys in breeding and nonbreeding seasons. However, ova from FSH-primed pr epubertal monkeys displayed greater development to blastocyst stage (54%) t han those from adult monkeys in the breeding season (16%) and nonbreeding s eason (0%); and ova from FSH-primed adult females in the breeding season ha d significantly (P < 0.05) greater developmental competence than those obta ined in the nonbreeding season (<greater than or equal to>morula stage, 54% vs, 3%; blastocyst stage, 16% vs. 0%), These data indicate that 1) rhesus monkey oocytes acquire developmental competence in a donor age-dependent ma nner, and 2) animal age and breeding season modulate the effect of FSH on o ocyte developmental competence in the rhesus monkey.