Water-soluble antioxidants inhibit macrophage recognition of oxidized erythrocytes

Citation
M. Beppu et al., Water-soluble antioxidants inhibit macrophage recognition of oxidized erythrocytes, BIOL PHAR B, 24(5), 2001, pp. 575-578
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
5
Year of publication
2001
Pages
575 - 578
Database
ISI
SICI code
0918-6158(200105)24:5<575:WAIMRO>2.0.ZU;2-U
Abstract
Effect of exogenously added water-soluble antioxidants on the mouse macroph age lectin-like receptor activity for oxidized erythrocytes was investigate d. A monolayer of thioglycollate-induced mouse peritoneal macrophages was p reincubated with each of the antioxidants at 37 degreesC for 1 h, and the b inding for mouse erythrocytes oxidized with ADP-chelated Fe(III) was examin ed. The binding was decreased by preincubation of macrophages with ascorbic acid-related compounds including ascorbic acid, erythorbic acid and dehydr oascorbic acid in a dose-dependent fashion at relatively high concentration s above 10 muM. The binding was similarly decreased by preincubation of mac rophages with catechin compounds including epicatechin, epigallocatechin, e picatechin gallate and epigallocatechin gallate in a dose-dependent fashion at 0.01-100 muM. The binding was more effectively decreased by preincubati on of macrophages with thiol-related compounds including glutathione, oxidi zed glutathione, glutathione Isopropyl ester. and N-acetylcysteine in a dos e dependent fashion at relatively low doses below 1 muM. These results show ed that water-soluble antioxidants especially glutathione and its derivativ es reduced the ability of macrophages to bind oxidized erythrocytes, sugges ting that the activity of lectin-like receptors of macrophages for oxidized erythrocytes was regulated by oxidative mechanisms.