Adenovirus-mediated p53 gene transfer to rat testis impairs spermatogenesis

Citation
M. Fujisawa et al., Adenovirus-mediated p53 gene transfer to rat testis impairs spermatogenesis, ARCH ANDROL, 46(3), 2001, pp. 223-231
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
ARCHIVES OF ANDROLOGY
ISSN journal
0148-5016 → ACNP
Volume
46
Issue
3
Year of publication
2001
Pages
223 - 231
Database
ISI
SICI code
0148-5016(200105/06)46:3<223:APGTTR>2.0.ZU;2-X
Abstract
The tumor suppressor protein p53 participates in normal cell differentiatio n as well as induction of programmed cell death. The authors investigated t he effect of p53 overexpression on spermatogenesis by transferring p53 gene into the rat testes. Replication-deficient recombinant adenovirus vectors were constructed to include cytomegalovirus (CMV) promoter driving wild-typ e p53 (Ad-CMV-p53) or beta -galactosidase (Ad-CMV-beta -gal). Virus was del ivered to cells of the tubules by slow retrograde injection through the ret e testis. At 0, 4. 7, and 14 days, testes were removed, weighed, and analyz ed histopathologically, including immunohistochemistry for p53, Bcl-2. Bar. and interleukin-1 beta converting enzyme (ICE). Testicular weight was decr eased in Ad-CMV-p53 group at 14 days after injection. while no change occur red in phosphate-buffered saline-injected controls or Ad-CMV-beta -gal-infe cted testes. Beyond 4 days, cell degradation in tubules interfered with imm unohistochemical observation in the Ad-CMV-p53 group. At 4 days, p53 was ex pressed mostly in spermatocytes. Bar showed greater expression in the p53 g roup than in the control or Ad-CMV-beta -gal group. ICE, expressed mostly i n spermatids. was more abundant in the p33 group than in controls. Overall, p53 overexpression in the testis impaired spermatogenesis.