Absence of estrogen receptor-alpha expression in human ovarian clear cell adenocarcinoma compared with ovarian serous, endometrioid, and mucinous adenocarcinoma

Citation
M. Fujimura et al., Absence of estrogen receptor-alpha expression in human ovarian clear cell adenocarcinoma compared with ovarian serous, endometrioid, and mucinous adenocarcinoma, AM J SURG P, 25(5), 2001, pp. 667-672
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
ISSN journal
0147-5185 → ACNP
Volume
25
Issue
5
Year of publication
2001
Pages
667 - 672
Database
ISI
SICI code
0147-5185(200105)25:5<667:AOEREI>2.0.ZU;2-D
Abstract
The mechanism that regulates growth in ovarian clear cell adenocarcinoma (C CA) is not well understood. A high incidence of concurrent endometriosis wi th CCA may indicate that estrogen is a growth promotor in CCA. To determine estrogen as a growth promotor, the authors investigated the presence or ab sence of estrogen receptor-alpha (ER-alpha), ER-beta, progesterone receptor . and dioxin receptor (i.e., aromatic hydrocarbon receptor) in clinically r esected ovarian CCA, serous adenocarcinoma (SAC), endometrioid adenocarcino ma (EAC), and mucinous adenocarcinoma (MAC) specimens using an immunohistoc hemical method. Expression of ER-alpha and ER-beta messenger ribonucleic ac id was examined by reverse transcription-polymerase chain reaction in three established CCA cell lines: KK, RMG-1. and MAC-II. None of the surgically resected CCA and CCA cell lines showed positive staining for ER-alpha. Conv ersely, 97.2% of SACs. 100% of EACs, and 70% of MACs showed positive nuclea r staining for ER-alpha (p <0.001). Conversely, positive ER-beta staining f or CCA (39.3%) was similar to that of SAC (41.7%) and MAC (30.0%). EAC (75% ) showed a higher expression of ER-beta (p <0.02). Progesterone receptor wa s detected in only 10.7% of CCA, compared with SAC and EAC (SAC, 86.1%; EAC , 91.7%: p <0.01). Aromatic hydrocarbon receptor was detected in all histol ogic types at an incidence of approximately 50% to 60%. Messenger ribonucle ic acid of ER-alpha and ER-beta was not detected in the three CCA cell line s. These findings indicate biologic characteristics that distinguish CCA fr om other types of ovarian epithelial cancer.