Column-switching high-performance liquid chromatographic determination of clarithromycin in human plasma with electrochemical detection

Citation
Sj. Choi et al., Column-switching high-performance liquid chromatographic determination of clarithromycin in human plasma with electrochemical detection, TALANTA, 54(2), 2001, pp. 377-382
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
TALANTA
ISSN journal
0039-9140 → ACNP
Volume
54
Issue
2
Year of publication
2001
Pages
377 - 382
Database
ISI
SICI code
0039-9140(20010412)54:2<377:CHLCDO>2.0.ZU;2-A
Abstract
A column-switching HPLC method was described for the direct analysis of cla rithromycin in human plasma using electrochemical detector without sample p re-purification step. Plasma samples were diluted with washing solvent, i.e . acetonirile-methanol-0.05 M potassium phosphate buffer (pH 7.0) (5:2:93, v/v) and then, injected to the precolumn. After plasma proteins had flowed out from the precolumn, clarithromycin and internal standard (roxithromycin ) were eluted to a Luna 2 C-18 column and separated with acetonitrile-metha nol-0.05 M potassium phosphate buffer (pH 7.0) (41:6:3, v/v). Electrochemic al oxidation of clarithromycin occurred at 0.87 V vs. Ag/AgCl reference ele ctrode with glassy carbon electrode. The calibration curve was linear in th e concentration range 0.1-4 mug ml(-1) with correlation coefficient of 0.99 8. This method showed excellent precision (RSD 3.5% at 0.1 mug ml(-1)) and accuracy (+/-2%) with the total analysis time per sample of 30 min. The pre sent method was successfully applied to the pharmacokinetic study of clarit hromycin in volunteers receiving a single oral administration of clarithrom ycin. (C) 2001 Elsevier Science B.V. Ali rights reserved.