An experimental comparison of electrospray ion-trap and matrix-assisted laser desorption/ionization post-source decay mass spectra for the characterization of small drug molecules

Citation
T. Leriche et al., An experimental comparison of electrospray ion-trap and matrix-assisted laser desorption/ionization post-source decay mass spectra for the characterization of small drug molecules, RAP C MASS, 15(8), 2001, pp. 608-614
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
0951-4198 → ACNP
Volume
15
Issue
8
Year of publication
2001
Pages
608 - 614
Database
ISI
SICI code
0951-4198(2001)15:8<608:AECOEI>2.0.ZU;2-2
Abstract
An experimental comparison of product ion spectra produced by matrix-assist ed laser desorption/ionization (MALDI) and electrospray ion-trap MSn for a group of small drug molecules is presented in this paper. The goal of the s tudy was to demonstrate the usefulness of MALDI-MS with post-source decay ( PSD) and collision-induced dissociation (CID) for the structural analysis o f small drug molecules in the drug discovery process, where traditionally e lectrospray LC/MS methods are used. PSD and PSD/CID gave diverse product io ns that were highly indicative of the structure of the drugs investigated ( a group of 4-quinolone antibiotics and oleandomycin). In addition, the numb er of different product ions generated with MALDI-MS was always higher than with electrospray ion-trap MS" (with n less than or equal to4) for the dru g molecules studied. This investigation also showed that the choice of a su itable MALDI matrix for the analysis of low molecular weight compounds is q uite important. It was found that of the three matrices examined, alpha -cy ano-4-hydroxycinnamic acid (alpha -CHCA) produced the most intense fragment ation levels while TiO2, with its advantage of virtually no low mass backgr ound signals, did not generate quite the same amount of information. Copyri ght (C) 2001 John Wiley & Sons, Ltd.