Melatonin acts as antioxidant and prooxidant in an organotypic slice culture model of Alzheimer's disease

Citation
Kl. Clapp-lilly et al., Melatonin acts as antioxidant and prooxidant in an organotypic slice culture model of Alzheimer's disease, NEUROREPORT, 12(6), 2001, pp. 1277-1280
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
12
Issue
6
Year of publication
2001
Pages
1277 - 1280
Database
ISI
SICI code
0959-4965(20010508)12:6<1277:MAAAAP>2.0.ZU;2-J
Abstract
An organotypic mouse brain slice culture system of Alzheimer's disease (AD) under low oxygen partial pressures was developed to determine the antioxid ant properties of the pineal hormone melatonin in vitro. Assays for biochem ical markers of oxidative stress including redox active iron assay, heme-ox ygenase-1 and 8-hydroxyguanosine immunoreactivity were performed along with morphological analysis for stressed tissue following amyloid-beta (A beta) 1-40 insult. Melatonin (100 muM) significantly reduced the appearance of c ondensed chromatin, redox active iron, heme-oxygenase-1 induction and 8-hyd roxyguanosine inmunoreactivity caused by 50 muM A beta. Melatonin also prev ented A beta -induced morphological signs of oxidative stress in tissue ult rastucture, including edema and dark degenerating profiles as visualized un der electron microscope. At elevated concentrations (1 mM), melatonin induc ed redox active iron and heme-oxgenase-1 immunoreactivity. Thus, while mela tonin may be a potential therapeutic agent in the prevention of oxidative s tress associated with A beta and AD, it can also induce markers of oxidativ e stress at millimolar concentrations. NeuroReport 12:1277-1280 (C) 2001 Li ppincott Williams & Wilkins.