p53 expression in the gastric mucosa before and after eradication of Helicobacter pylori

Citation
K. Satoh et al., p53 expression in the gastric mucosa before and after eradication of Helicobacter pylori, HELICOBACT, 6(1), 2001, pp. 31-36
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
HELICOBACTER
ISSN journal
1083-4389 → ACNP
Volume
6
Issue
1
Year of publication
2001
Pages
31 - 36
Database
ISI
SICI code
1083-4389(200103)6:1<31:PEITGM>2.0.ZU;2-T
Abstract
Background. Accumulation of p53 has been recognized in the gastric mucosa i nfected with Helicobacter pylori. We investigated the prevalence of p53-pos itive cells in the gastric mucosa before and one month after eradication of H. pylori and the relationship between p53 positivity and inflammation and cell proliferation. Methods. The subjects included 24 H. pylori-posit ive patients. They achiev ed eradication one month after anti-H. pylori therapy. Biopsies were taken from the greater curvatures of the antrum and middle body. H. pylori status was assessed using culture and tissue section (Giemsa stain). Serial secti ons were used for examination of gastritis (hematoxylin and eosin stain) an d for immunostaining of p53, Ki-67 and myeloperoxidase (MPO). p53 index and Ki-67 labeling index (LI) were calculated by counting p53-positive and Ki- 67-positive cells in the entire gastric pits longitudinally sectioned and e xpressing them as a percentage of the total cells in a gastric pit. In the neck regions with and without p53-positive cells, polymorphonuclear leukocy tes (PMNs) were counted in the corresponding area (/50x50 mum(2)) of the se ctions stained both with p53 and MPO. Results. p53-positive cells decreased significantly after eradication of H. pylori. Before eradication, the number of PMNs was significantly higher in the neck regions with p53-positive cells than in those without. Conclusions. In the gastric mucosa infected with H. pylori, p53-positive ce lls were found in the neck region infiltrated with PMNs. p53 expression dec reased significantly one month after eradication of H. pylori.