cDNA sequence and genomic structure of the rat RET proto-oncogene

Citation
I. Matera et al., cDNA sequence and genomic structure of the rat RET proto-oncogene, DNA SEQ, 11(5), 2000, pp. 405-417
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
DNA SEQUENCE
ISSN journal
1042-5179 → ACNP
Volume
11
Issue
5
Year of publication
2000
Pages
405 - 417
Database
ISI
SICI code
1042-5179(2000)11:5<405:CSAGSO>2.0.ZU;2-U
Abstract
The RET proto-oncogene, a member of the Receptor Tyrosine Kinase family, pl ays a crucial role during the development of the excretory system and the e nteric nervous system, as demonstrated by in vivo animal studies and by its involvement in the pathogenesis of several human neurocristopathies like H irschsprung disease and Multiple Endocrine Neoplasia type 2. Using a multis tep RT-PCR approach we have isolated and sequenced the cDNA of the whole ra t RET proto-oncogene, reporting the deduced amino acid sequence in comparis on with the human and mouse counterparts. Moreover, two different isoforms (RET9 and RET51) have been confirmed in the rat, while a third RET isoform demonstrated in human (RET43) has not resulted to be conserved in this spec ies. Finally, we have determined the genomic structure of the rat RET proto -oncogene comparing the exon-intron boundaries and intron sizes with the kn own structure of the human homologous gene. Our findings will facilitate th e molecular study of appropriate rat models of RET related human diseases.