Decreased natural killer (NK) cell function in chronic NK cell lymphocytosis associated with decreased surface expression of CD11b

Citation
Js. Orange et al., Decreased natural killer (NK) cell function in chronic NK cell lymphocytosis associated with decreased surface expression of CD11b, CLIN IMMUNO, 99(1), 2001, pp. 53-64
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
CLINICAL IMMUNOLOGY
ISSN journal
1521-6616 → ACNP
Volume
99
Issue
1
Year of publication
2001
Pages
53 - 64
Database
ISI
SICI code
1521-6616(200104)99:1<53:DNK(CF>2.0.ZU;2-3
Abstract
Chronic natural killer cell lymphocytosis (CNKL) is characterized by greatl y increased numbers of natural killer (NK) cells and patients with this dis ease may survive for long periods. This is in contrast to patients with leu kemic proliferations of NK cells who can have a rapidly progressive clinica l course. We identified a pediatric patient who was largely healthy who had CNKL and we sought to determine if the expanded CD16(+)CD3(-) population i n this patient functions differently than classical Mt cells. Cytotoxic act ivity against NK cell-sensitive K562 target cells was present, but lower th an that in control donors when calculated as lytic units per CD16+CD3- cell . This cytolytic activity was inducible in patient samples by IL-2/IL-12 st imulation proportionately to that induced in samples from control donors. I ntracellular perforin was also present and induced in patient CD16(+)CD3(-) cells similarly to controls. Other presumed NK cell activities, such as IL -2/IL-12 induced IFN-gamma expression and initiation of apoptosis evidenced by annexin V binding after CD16 crosslinking were present in patient sampl es. Patient CD16+CD3- cells, however, differed from classical NK cells, as the majority did not express CD56, CD57, CD8, or CD11b. Most convincingly, there was a 5 log decrease in CD11b expression in patient CD16(+)CD3(-) cel ls compared to control as determined by mean channel fluorescence. These ob served differences may explain the relatively benign phenotype of this diso rder. (C) 2001 Academic Press.