Proliferating cell nuclear antigen and epidermal growth factor receptor (EGFr) status in renal cell carcinoma patients with polysomy of chromosome 7

Citation
Ps. Amare et al., Proliferating cell nuclear antigen and epidermal growth factor receptor (EGFr) status in renal cell carcinoma patients with polysomy of chromosome 7, CANC GENET, 125(2), 2001, pp. 139-146
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER GENETICS AND CYTOGENETICS
ISSN journal
0165-4608 → ACNP
Volume
125
Issue
2
Year of publication
2001
Pages
139 - 146
Database
ISI
SICI code
0165-4608(200103)125:2<139:PCNAAE>2.0.ZU;2-A
Abstract
We investigated 40 cases of renal cell carcinoma (RCC) to study the polysom y 7 status in papillary and clear-cell types (nonpapillary RCC) and relatio nship with clinical, pathological, and biological features such as grade, s tage, tumor proliferation rate (PCNA expression) and epidermal growth facto r receptor (EGFr) expression and thereby to understand the prognostic signi ficance of polysomy 7 and EGFr expression. In a prospective study, chromoso me 7 copy number was analyzed in tumor cells by using fluorescence in situ hybridization (FISH) with an alpha -satellite DNA probe for chromosome 7. B oth proliferating cell nuclear antigen (PCNA) and EGFr expression were exam ined in paraffin sections by immunostaining. The relationship between clini copathological and clinicobiological parameters was evaluated by appropriat e statistical methods. Polysomy 7 was present in 100% of papillary and 56.2 % of clear-cell types RCC. In clear-cell RCC; in comparison with polysomy 7 -dominant (D) category (20-50% polysomy-7 cells), polysomy 7-major (M) cate gory (>50% polysomy 7 cells) was associated with higher tumor grade (P = 0. 05). Polysomy 7 was also correlated with stage of the disease (P = 0.006). The PCNA index ranged between 12.8-89.6% and was comparatively high in high -grade tumors (P = 0.001). The PCNA index was also correlated with polysomy 7 (P = 0.002), and the association was stronger in tumors with polysomy M versus polysomy D category (P = 0.02). The EGFr expression did not correlat e with either grade, stage, PCNA, or polysomy 7. The correlation of polysom y 7 with less favorable prognostic factors such as higher tumor grade, stag e, and higher proliferative index in the present study indicates that polys omy 7 might be used as a prognostic predictor in clear-cell RCC. Evaluation of clinical end points will confirm the prognostic potential of the geneti c marker polysomy 7 in our study. (C) 2001 Elsevier Science Inc. All rights reserved.