Hypogin, a novel antifungal peptide from peanuts with sequence similarity to peanut allergen

Authors
Citation
Xy. Ye et Tb. Ng, Hypogin, a novel antifungal peptide from peanuts with sequence similarity to peanut allergen, J PEPT RES, 57(4), 2001, pp. 330-336
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF PEPTIDE RESEARCH
ISSN journal
1397-002X → ACNP
Volume
57
Issue
4
Year of publication
2001
Pages
330 - 336
Database
ISI
SICI code
1397-002X(200104)57:4<330:HANAPF>2.0.ZU;2-K
Abstract
A protein designated hypogin, with a prominent suppressive action on the gr owth of the fungi Mycosphaerella arachidicola, Fusarium oxysporum and Copri nus comatus, was isolated from seeds of the peanut Arachis hypogaea. The pr otein inhibited human immunodeficiency virus (HIV) reverse transcriptase an d enzymes associated with HIV infection including alpha -glucosidase and be ta -glucosidase. The proliferative response of mouse splenocytes was attenu ated in the presence of the protein. The protein exhibited a molecular mass of 7.2 kDa in tricine gel electrophoresis and gel filtration on Superdex 7 5 and an N-terminal sequence resembling peanut allergen Ara Hf. The isolati on procedure involved affinity chromatography on Affi-gel blue gel and ion- exchange chromatography on CM-Sepharose. The protein was adsorbed in both c hromatographic media.