A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control

Citation
Kd. Bodily et al., A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control, J NEUROSC, 21(9), 2001, pp. 3113-3125
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE
ISSN journal
0270-6474 → ACNP
Volume
21
Issue
9
Year of publication
2001
Pages
3113 - 3125
Database
ISI
SICI code
0270-6474(20010501)21:9<3113:ANMOTI>2.0.ZU;2-J
Abstract
We describe here the cloning and functional characterization of a neural-sp ecific novel member of the Ig superfamily, turtle (tutl), with a structure of five Ig C2-type domains, two fibronectin type III domains, and one trans membrane region. Alternative splicing of the tutl gene produces at least fo ur Tutl isoforms, including two transmembrane proteins and two secreted pro teins, with primary structures closely related to a human brain protein (KI AA1355), the Deleted in Colorectal Cancer/Neogenin/ Frazzled receptor famil y, and the Roundabout/Dutt1 receptor family. An allelic series of tutl gene mutations resulted in recessive lethality to semilethality, indicating tha t the gene is essential. In contrast to other family members, tutl does not play a detectable role in axon pathfinding or nervous system morphogenesis . Likewise, basal synaptic transmission and locomotory movement are unaffec ted. However, tutl mutations cause striking movement defects exhibited in s pecific types of highly coordinated behavior. Specifically, tutl mutants di splay an abnormal response to tactile stimulation, the inability to regain an upright position from an inverted position (hence, "turtle"), and the in ability to fly in adulthood. These phenotypes demonstrate that tutl plays a n essential role in establishing a nervous system capable of executing coor dinated motor output in complex behaviors.