The expression pattern of the cell cycle inhibitor p19(INK4d) by progenitor cells of the rat embryonic telencephalon and neonatal anterior subventricular zone

Citation
V. Coskun et Mb. Luskin, The expression pattern of the cell cycle inhibitor p19(INK4d) by progenitor cells of the rat embryonic telencephalon and neonatal anterior subventricular zone, J NEUROSC, 21(9), 2001, pp. 3092-3103
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE
ISSN journal
0270-6474 → ACNP
Volume
21
Issue
9
Year of publication
2001
Pages
3092 - 3103
Database
ISI
SICI code
0270-6474(20010501)21:9<3092:TEPOTC>2.0.ZU;2-K
Abstract
In this study we investigated whether the pattern of expression of the cycl in-dependent kinase inhibitor p19(INK4d) by the unique progenitor cells of the neonatal anterior subventricular zone (SVZa) can account for their abil ity to divide even though they express phenotypic characteristics of differ entiated neurons. p19(INK4d) was chosen for analysis because it usually act s to block permanently the cell cycle at the G(1) phase. p19(INK4d) immunor eactivity and the incorporation of bromodeoxyuridine (BrdU) by SVZa cells w ere compared with that of the more typical progenitor cells of the prenatal telencephalic ventricular zone. In the developing telencephalon, p19(INK4d ) is expressed by postmitotic cells and has a characteristic perinuclear di stribution depending on the laminar position and state of differentiation o f a cell. Moreover, the laminar-specific staining of the developing cerebra l cortex revealed that the ventricular zone (VZ) is divided into p19(INK4d) (+) and p19(INK4d) (-) sublaminae, indicating that the VZ has a previously unrecognized level of functional organization. Furthermore, the rostral mi gratory stream, traversed by the SVZa-derived cells, exhibits an anterior ( high)-posterior(low) gradient of p19(INK4d) expression. On the basis of the p19(INK4d) immunoreactivity and BrdU incorporation, SVZa-derived cells app ear to exit and reenter the cell cycle successively. Thus, in contrast to t elencephalic VZ cells, SVZa cells continue to undergo multiple rounds of di vision and differentiation before becoming postmitotic.