Polymorphism and allelic loss at the AS3 locus on 13q12-13 in esophageal squamous cell carcinoma

Citation
H. Harada et al., Polymorphism and allelic loss at the AS3 locus on 13q12-13 in esophageal squamous cell carcinoma, INT J ONCOL, 18(5), 2001, pp. 1003-1007
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
18
Issue
5
Year of publication
2001
Pages
1003 - 1007
Database
ISI
SICI code
1019-6439(200105)18:5<1003:PAALAT>2.0.ZU;2-D
Abstract
Our previous studies of esophageal squamous cell carcinoma (ESC) revealed t hat frequent loss of heterozygosity (LOH) on 13q12-13 was associated with l ymph node metastasis. These results suggest that 13q12-13 may harbor a tumo r suppressor gene which regulates lymph node metastasis. Recently, a new tu mor suppressor candidate, AS3, was identified in this region. We therefore examined AS3 alterations by PCR-SSCP and RT-PCR. Although polymorphisms wer e found in exon 10 and introns 21, 23, 25 and 29, a possible tumor specific mutation in exon G was identified in only one of 31 ESC-derived cell lines , indicating that the remaining allele of AS3 was rarely inactivated by mut ational events. Interestingly, ESC patients with rare alleles frequently ex hibited lymph node metastasis. These results suggest that polymorphic varia tion and LOH at the AS3 locus might be associated with lymph node involveme nt in ESC.