SMRT as a T3SF-binding protein

Citation
K. Onishi et al., SMRT as a T3SF-binding protein, INT J ONCOL, 18(5), 2001, pp. 985-989
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
18
Issue
5
Year of publication
2001
Pages
985 - 989
Database
ISI
SICI code
1019-6439(200105)18:5<985:SAATP>2.0.ZU;2-1
Abstract
p53-binding consensus-like sequence (T3SF) is located in the murine promote r region of tissue inhibitor of metalloproteinase 3 gene. To identify the g enes that encode proteins that bind to T3SF DNA sequence, we screened a cDN A library using the Southwestern technique. The SMRT gene was cloned as one of the candidates. Addition of antibody against SMRT reduced the intensity of a band that is supposed to contain SMRT in electrophoresis mobility shi ft assay, although antibody against p53 had no effect. Ultraviolet (UV)-irr adiation reduced the intensity of the SMRT complex whereas p53 complex was stabilized by UV-irradiation. These results suggest that SMRT may bind to T 3SF sequence in p53-independent manner and dissociate from the sequence by UV irradiation.