Expression of the chemokine receptor CCR3 on human mast cells

Citation
A. De Paulis et al., Expression of the chemokine receptor CCR3 on human mast cells, INT A AL IM, 124(1-3), 2001, pp. 146-150
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
ISSN journal
1018-2438 → ACNP
Volume
124
Issue
1-3
Year of publication
2001
Pages
146 - 150
Database
ISI
SICI code
1018-2438(200101/03)124:1-3<146:EOTCRC>2.0.ZU;2-I
Abstract
Background: The aim of this study was to investigate whether human mast cel ls express functional active CCR3 receptors, which are activated by CC chem okines. These ligands include the CCR3-selective chemokines eotaxin and eot axin-2 and the more promiscuous CC chemokines, MCP-4, MCP-3, MCP-2 and RANT ES. Methods: Immunohistochemical analysis was performed on skin, gut and lu ng specimens. Double immunostaining was performed with anti-CCR3 and antitr yptase, and anti-CCR3 and antichymase antibody (Ab) by using the avidin-bio tin-peroxidase system with two different substrates. Mast ce[is were isolat ed and purified from human lung parenchyma (HLMC) by countercurrent elutria tion followed by discontinuous Percoll density gradient. Flow-cytometric an alysis of HLMC surface CCR3 expression was performed with the monoclonal Ab anti-CCR3 (7B11). Functional activation of H LMC was verified by th e abil ity of cells to release histamine and/or migrate in response to eotaxin. Re sults: High percentages (>70%) of tryptase-positive cells showing CCR3 expr ession were found in the skin and in the intestinal submucosa, whereas much lower percentages (less than or equal to 20%) were found in the intestinal mucosa and in the lung interstitium. Eotaxin (1-100 nM) neither induced hi stamine release from HLMC nor enhanced anti-IgE-induced histamine release. In contrast, eotaxin (10-100 nM) and RANTES (10-100 nM) induced HLMC chemot axis in vitro. Preincubation of HLMC with antibody anti-CCR3 (5 mug/ml) bef ore loading into the chemotaxis chamber abrogated chemotaxis elicited by eo taxin. Double immunostaining with anti-CCR3 and anti-chymase antibody showe d that the vast majority of CCR3-expressing mast cells in the Various human tissues examined were tryptase-chymase double-positive. Conclusions: These results indicate that CCR3 is expressed on human mast cells and that these cells are attracted by CCR3-binding chemokines. Copyright (C) 2001 S. Karg er AG, Basel.