Characterization of proteins in the outer membrane preparation of a murinepathogen, Helicobacter bilis

Citation
Zm. Ge et al., Characterization of proteins in the outer membrane preparation of a murinepathogen, Helicobacter bilis, INFEC IMMUN, 69(5), 2001, pp. 3502-3506
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
0019-9567 → ACNP
Volume
69
Issue
5
Year of publication
2001
Pages
3502 - 3506
Database
ISI
SICI code
0019-9567(200105)69:5<3502:COPITO>2.0.ZU;2-U
Abstract
Helicobacter bills is a bacterial pathogen associated viith multifocal hepa titis and inflammatory bowel disease in certain strains of mice. This bacte rium colonizes the liver, bile, and lower intestine in mice and has also be en isolated from a wide spectrum of laboratory animals. In this study, prot eins present in the outer membrane preparation (OMP) of four H, bilis strai ns isolated from a mouse. a dog, a rat, and a gerbil were characterized and compared with that of Helicobacter pylori, a human gastric pathogen, all f oul H, bilis strains had similar OMP protein profiles that were distinct fr om those of H, pylori. Immunoblotting demonstrated that OMP proteins from H . bilis and H, pylori have Little cross-reactivity, except for their flagel lins. Nine major immunogenic polypeptides were present in the H. bills OMPs . By using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electr ophoresis, five heat-modifiable proteins with molecular masses of 82, 66, 5 2, 47 and 37 kDa were identified. The N-terminal sequences of the 46- end 4 7-kDa OMP proteins and no homology with protein sequences available in publ ic databases. These results indicate that H, bilis has a conserved, unique OMP protein profile that is distinct from those of H, pylori.