BACKGROUND. The clinicopathologic characteristics of breast carcinoma with
chromosomal aneusomy detected by fluorescence in situ hybridization (FISH)
have yet to be clarified.
METHODS. Fine-needle aspiration biopsy (FNAB) samples were obtained from 11
3 breast tumors and were subjected to FISH analysis using centromeric probe
s for chromosomes 1, 11, and 17 to study a numerical aberration of these ch
romosomes and its correlation with various clinicopathologic features of br
RESULTS. Polysomy was observed in 77.0%, 50.5%, and 37.2% of breast carcino
ma samples for chromosomes I, It, and 17, respectively, and monosomy was ob
served in 1.8%, 8.8%, and 22.1% for chromosomes 1, 11, and 17, respectively
. High histologic grade showed a significant correlation (P < 0.05) with po
lysomy of chromosome 11. Lymph node metastasis showed a significant correla
tion (P 0.05) with polysomy of all three chromosomes, and positivity of lym
ph node metastasis increased as the number of polysomic chromosomes increas
ed. In addition, estrogen receptor negativity was correlated significantly
(P < 0.05) with monosomy of chromosome 17, and progesterone receptor negati
vity was correlated significantly (P < 0.05) with polysomy of chromosomes 1
1 and 17.
CONCLUSIONS, Aneusomy of chromosome 1, 11, or 17 detected by FISH is correl
ated significantly with various clinicopathologic features of breast carcin
oma. Because FISH analysis of chromosomal aneusomy can be done using FNAB s
amples, this technique seems to have the potential to be used for a better,
preoperative definition of the biologic characteristics of breast tumors.
Cancer (Cancer Cytopathol) 2001;93:165-170. (C) 2001 American Cancer Societ