Identification and quantitation of steroid hormones in marine gastropods by GC/MS

Citation
M. Lu et al., Identification and quantitation of steroid hormones in marine gastropods by GC/MS, BUNSEKI KAG, 50(4), 2001, pp. 247-255
Citations number
18
Language
GIAPPONESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
BUNSEKI KAGAKU
ISSN journal
0525-1931 → ACNP
Volume
50
Issue
4
Year of publication
2001
Pages
247 - 255
Database
ISI
SICI code
0525-1931(200104)50:4<247:IAQOSH>2.0.ZU;2-P
Abstract
In order to clarify the effect of the contamination of organotin compounds in a marine environment oil the imposex of gastropods, the speciation and l evels of steroid hormones of the organisms were investigated by means of th e GC/MS method. Testosterone and 17 beta -estradiol were first identified a nd quantitated in the testes and ovaries of Thais clavigera, respectively. In addition, androsterone, estrone and ethynlestradiol were also found in t he respective tissues of Babylonia japonica. The extraction and refining pr ocedures were constructed, and the passage of C-8-SPE was quite effective f or clean-up of the steroid fractions. The recovery of testosterone and 17 b eta -estradiol through the whole procedure were examined by adding a surrog ate compound, 17 beta -estradiol-d(3). Good recoveries of between 83% ana 9 6% were obtained. Based on these, the concentrations of testosterone in the testes and 17 beta -estradiol in the ovaries of both marine gastropods wer e well determined. The testes of Babylonia japonica were stained both histo logically and immunohistochemically to observe the presence of inter- stiti al cells, testosterone producing cells known in mammals.