Endothelin ETA and ETB receptor antagonism during cold preservation in renal transplantation

Citation
Ft. Hammad et al., Endothelin ETA and ETB receptor antagonism during cold preservation in renal transplantation, TRANSPLANT, 71(5), 2001, pp. 619-627
Citations number
64
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
TRANSPLANTATION
ISSN journal
0041-1337 → ACNP
Volume
71
Issue
5
Year of publication
2001
Pages
619 - 627
Database
ISI
SICI code
0041-1337(20010315)71:5<619:EEAERA>2.0.ZU;2-I
Abstract
Background. In this study, we investigated the effects of selective endothe lin (ET) receptor antagonism during different periods of cold ischemia on g lomerular and tubular function and long-term survival in renal transplantat ion. Methods. Left renal transplantation was performed in Lewis rats after 2 hr of cold ischemia without (n=8) and with (n=6) ETA receptor antagonism and a fter 16 hr of cold ischemia without treatment (n=6), with ETA receptor anta gonism (n=8) and with ETB receptor antagonism (n=6). A control group (n=8) underwent right nephrectomy and left renal denervation. The ETA and ETB rec eptor antagonists (BQ-610 and A-192621, respectively) were added to the pre servation solution (EuroCollins), After transplantation, renal glomerular a nd tubular functions were monitored for up to 60 days or death. Results. All animals in the control and 2-hr groups survived the follow-up protocol, with early postoperative recovery of glomerular and tubular funct ion while the entire untreated 16-hr group died between day 3-6 postoperati vely, BQ-610 treatment had no measurable effect on the renal function in th e 2-hr group, however, it improved glomerular and tubular functions and led to 50% long-term survival (60 days) in the 16-hr group. A-192621 treatment had no effect on long-term survival or renal parameters. Conclusion. ETA receptor antagonism had protective renal effects after prol onged ischemic preservation in renal transplantation while ETB receptor ant agonism had not.