Use of amino-carbonyl reaction and chemiluminescence detection to the flow-injection determination of some amino acids

Citation
J. Michalowski et A. Kojlo, Use of amino-carbonyl reaction and chemiluminescence detection to the flow-injection determination of some amino acids, TALANTA, 54(1), 2001, pp. 107-113
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
TALANTA
ISSN journal
0039-9140 → ACNP
Volume
54
Issue
1
Year of publication
2001
Pages
107 - 113
Database
ISI
SICI code
0039-9140(20010330)54:1<107:UOARAC>2.0.ZU;2-7
Abstract
Some amino acids were found to react with carbonyl functional groups of hum ic acid. Products of this reaction give strong chemiluminescence during the ir oxidation with N-bromosuccinimide (NBS) in alkaline solution. Humic acid s from different sources produced similiar signal magnitude. This effect wa s employed to the flow-injection determination of glycine and arginine in p harmaceutical formulations with considerable selectivity against different amino compounds. The proposed method is fast and simple. Detection limit is 0.20 and 0.25 mg.l(-1) for glycine and arginine respectively, and 115 samp les per h can be determined. (C) 2001 Elsevier Science B.V. All rights rese rved.