Lifestyles and mental health status are associated with natural killer cell and lymphokine-activated killer cell activities

Citation
K. Morimoto et al., Lifestyles and mental health status are associated with natural killer cell and lymphokine-activated killer cell activities, SCI TOTAL E, 270(1-3), 2001, pp. 3-11
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology
Journal title
SCIENCE OF THE TOTAL ENVIRONMENT
ISSN journal
0048-9697 → ACNP
Volume
270
Issue
1-3
Year of publication
2001
Pages
3 - 11
Database
ISI
SICI code
0048-9697(20010410)270:1-3<3:LAMHSA>2.0.ZU;2-Q
Abstract
We investigated the association of lifestyle and mental health status with natural killer (NK) cell and lymphokine-activated killer (LAK) cell activit ies in healthy males. NK cell activity was determined in 105 male workers a nd LAK cell activity was determined in 54 male workers, peripheral blood wa s obtained from each subject and peripheral blood mononuclear cells (PBMC) were isolated from the blood. These PBMC were used as effector cells. LAK c ells were generated by incubation of PBMC with interleukin-2 for 72 h. NK c ell activity against NK-sensitive K562 cells and LAK cell activity against NK-resistant Raji cells were examined by Cr-51 release assay. Overall lifes tyles were evaluated according to the answers on a questionnaire regarding eight health practices (cigarette smoking, alcohol consumption, eating brea kfast, hours of sleep, hours of work, physical exercise, nutritional balanc e, mental stress). Subjects with a good overall lifestyle showed significan tly higher NK cell (P< 0.05) and LAK cell (P < 0.05) activities than those with a poor overall lifestyles. Among eight lifestyle factors, cigarette sm oking has relatively strong effects on NK cell and LAK cell activities. Sub jects who complained of unstable mental status had significantly lower NK c ell activity than those who reported stable mental status. When subjects we re divided into four groups by lifestyle and mental health status, subjects who had poor or moderate lifestyle and reported unstable mental status sho wed the lowest NK cell activity and subjects who had good lifestyle and rep orted stable mental status showed the highest NK cell activity among four g roups. (C) 2001 Elsevier Science B.V. All rights reserved.