Noninvasive functional optical spectroscopy of human breast tissue

Citation
N. Shah et al., Noninvasive functional optical spectroscopy of human breast tissue, P NAS US, 98(8), 2001, pp. 4420-4425
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
98
Issue
8
Year of publication
2001
Pages
4420 - 4425
Database
ISI
SICI code
0027-8424(20010410)98:8<4420:NFOSOH>2.0.ZU;2-5
Abstract
Near infrared diffuse optical spectroscopy and diffuse optical imaging are promising methods that eventually may enhance or replace existing technolog ies for breast cancer screening and diagnosis. These techniques are based o n highly sensitive, quantitative measurements of optical and functional con trast between healthy and diseased tissue. In this study, we examine whethe r changes in breast physiology caused by exogenous hormones, aging, and flu ctuations during the menstrual cycle result in significant alterations in b reast tissue optical contrast. A noninvasive quantitative diffuse optical s pectroscopy technique, frequency-domain photon migration, was used. Measure ments were performed on 14 volunteer subjects by using a hand-held probe. I ntrinsic tissue absorption and reduced scattering parameters were calculate d from frequency-domain photon migration data. Wavelength-dependent absorpt ion (at 674, 803, 849, and 956 nm) was used to determine tissue concentrati on of oxyhemoglobin, deoxyhemoglobin, total hemoglobin, tissue hemoglobin o xygen saturation, and bulk water content. Results show significant and dram atic differences in optical properties between menopausal states. Average p remenopausal intrinsic tissue absorption and reduced scattering values at e ach wavelength are 2.5- to 3-fold higher and 16-28% greater, respectively, than absorption and scattering for postmenopausal subjects. Absorption and scattering properties for women using hormone replacement therapy are inter mediate between premenopausal and postmenopausal populations. Physiological properties show differences in mean total hemoglobin (7.0 muM, 11.8 muM, a nd 19.2 muM) and water concentration relative to pure water (10.9%, 15.3%, and 27.3%) for postmenopausal, hormone replacement therapy, and premenopaus al subjects, respectively. Because of their unique, quantitative informatio n content, diffuse optical methods may play an important role in breast dia gnostics and improving our understanding of breast disease.