Feasible production of camptothecin by hairy root culture of Ophiorrhiza pumila

Citation
K. Saito et al., Feasible production of camptothecin by hairy root culture of Ophiorrhiza pumila, PL CELL REP, 20(3), 2001, pp. 267-271
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
20
Issue
3
Year of publication
2001
Pages
267 - 271
Database
ISI
SICI code
0721-7714(200103)20:3<267:FPOCBH>2.0.ZU;2-1
Abstract
Camptothecin derivatives are used clinically as anti-tumor alkaloids. Campt othecin and its related compounds are at present obtained by extraction fro m intact plants, but transformed plant cell cultures may be an alternative source of production. We have established a hairy root culture of Ophiorriz a pumila (Rubiaceae) transformed by Agrobacterium rhizogenes strain 15834. This hairy root culture grew well, increasing by 16-fold during 5 weeks in liquid culture, and it produced camptothecin as a main alkaloid up to 0.1% per dry weight of the cells. Interestingly, not only the hairy root cells c ontained camptothecin, but the culture medium also accumulated substantial amounts. Camptothecin content in the medium was increased by the presence o f a polystyrene resin (Diaion HP-20) that absorbed camptothecin. Camptothec in was easily recovered from the resin. Our method is the most feasible and commercially applicable way to produce camptothecin by in vitro cell cultu re.