Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants

Citation
Zy. Wang et al., Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants, PL CELL REP, 20(3), 2001, pp. 213-219
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
20
Issue
3
Year of publication
2001
Pages
213 - 219
Database
ISI
SICI code
0721-7714(200103)20:3<213:EOASSA>2.0.ZU;2-V
Abstract
Transgenic tall fescue (Festuca arundinacea Schreb.) plants have been gener ated that express foreign genes encoding a rumen-stable protein rich in sul phur-containing amino acids. The aim was to improve the protein quality of a forage grass for ruminant nutrition. Chimeric sulphur-rich sunflower albu min (SFA8) genes, including an endoplasmic reticulum retention signal (KDEL ), were constructed under the control of constitutive (CaMV 35S) and light- regulated (wheat Cab) promoters. These constructs were introduced into the tall fescue genome by microprojectile bombardment of embryogenic suspension cells. The sunflower albumin transgenes stably integrated into the plant g enome as demonstrated by Southern hybridization analysis. The transgenic ta ll fescue plants produced the expected transcript, and the corresponding su lphur-rich SFA8 protein accumulated up to 0.2% of the total soluble protein in individual transgenic plants.