Altered fructan accumulation in transgenic Lolium multiflorum plants expressing a Bacillus subtilis sacB gene

Citation
Xd. Ye et al., Altered fructan accumulation in transgenic Lolium multiflorum plants expressing a Bacillus subtilis sacB gene, PL CELL REP, 20(3), 2001, pp. 205-212
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
20
Issue
3
Year of publication
2001
Pages
205 - 212
Database
ISI
SICI code
0721-7714(200103)20:3<205:AFAITL>2.0.ZU;2-M
Abstract
Ryegrasses, like many C3 plants, accumulate fructan, which plays an importa nt role in assimilate partitioning, as the major non-structural storage car bohydrate. The present study describes the transformation of a Bacillus sub tilis sacB gene, with vacuolar targeting signal sequences and driven by con stitutive promoters, into Italian ryegrass (Lolium multiflorum Lam.) by mic roprojectile bombardment of embryogenic suspension cells. The expression of the chimeric sacB genes in transgenic ryegrass plants and the concomitant accumulation of low levels of bacterial levan were found to substantially d istort the native grass fructan synthesis pattern. High-molecular-weight na tive fructan was depleted, and the pattern of accumulation of oligosacchari des in the range of 5-35 degree of polymerization was altered. The levan-ac cumulating sacB-transgenic ryegrass plants had a lower level of total fruct ose, unchanged sucrose levels, and slightly reduced hexose levels compared to the isogenic controls. Growth of the levan-accumulating sacB-transgenic ryegrass plants slowed down with the onset of the reproductive phase. Flowe ring plants were stunted and had narrower leaves and poorly developed roots . The association between the manipulated fructan metabolism and the phenot ype of the levan-accumulating sacB-transgenic ryegrass plants is discussed.